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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/825

Title: Direct Conjugation of Semiconductor Nanocrystals to a Globular Protein to Study Protein-Folding Intermediates
Authors: Sarkar, Rupa
Narayanan, S Shankara
Pa°lsson, Lars-Olof
Dias, Fernando
Monkman, Andy
Pal, Samir Kumar
Issue Date: 2007
Publisher: J. Phys. Chem. B
Citation: R. Sarkar, S. S. Narayanan, L. –O. Pa°lsson, F. Dias, A. Monkman and S. K. Pal, Direct Conjugation of semiconductor nanocrystals to a globular protein to study protein-folding intermediates, J. Phys. Chem. B 111 (2007) 12294.
Abstract: In this Article, we study the development of semiconductor nanocrystals (quantum dots of average diameter less than 2 nm) directly conjugated to a transporter protein human serum albumin (HSA) as fluorescent biological labels. Fo¨rster resonance energy transfer (FRET) from the amino acid tryptophan (Trp214) to quantum dot in HSA is monitored to follow the local and global changes in the protein structure during thermal unfolding and refolding processes. This study is likely to attract widespread attention as a powerful tool for the study of protein folding.
URI: http://hdl.handle.net/123456789/825
Appears in Collections:2007

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