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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/81

Title: Ultrafast Spectroscopic Study on Caffeine Mediated Dissociation of Mutagenic Ethidium from Synthetic DNA and Various Cell Nuclei
Authors: Banerjee, Soma
Bhowmik, Debajit
Verma, Pramod K
Mitra, Rajib K
Sidhhanta, Anirban
Basu, Gautam
Pal, Samir K
Issue Date: 2011
Publisher: J. Phys. Chem. B
Citation: S. Banerjee, D. Bhowmik, P. K. Verma, R. K. Mitra, A. Siddhanto, G. Basu and S. K. Pal, Ultrafast Spectroscopic Study on Caffeine Mediated Dissociation of Mutagenic Ethidium from Synthetic DNA and Various Cell Nuclei, J. Phys. Chem. B, 2011, 115, 14776–14783
Abstract: We report a systematic investigation of caffeine-induced dissociation of ethidium (Et) cation, a potential mutagen. Time-resolved fluorescence studies are consistent with a mechanism where caffeine Et complex formation in bulk solution drives the dissociation ofDNA-bound Et. Temperature-dependent picosecond-resolved studies show the caffeine Et complex to be stable over a wide range of temperature, within and beyond the normal physiological limit. A combination of NMR spectroscopy and dynamic light scattering experiments allowed us to propose a molecular model of the caffeine Et complex. Caffeine-induced extraction of Et from whole cells was also performed on squamous epithelial cells collected from the inner lining of the human mouth, A549 (lung carcinoma), A375 (human skin), RAW (macrophage), and Vero (African green monkey kidney epithelium) cell lines. Interestingly the efficiency of caffeine in extracting Et has been found to be dependent on cell types. Our results both in vitro as well as ex vivo provide important clues about the efficiency and mechanism of caffeine as a potential antimutagenic therapeutic agent.
URI: http://hdl.handle.net/123456789/81
Appears in Collections:2011

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