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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/360

Title: A versatile fiber-optic coupled system for sensitive optical spectroscopy in strong ambient light
Authors: Sinha, Sudarson Sekhar
Verma, Pramod Kumar
Makhal, Abhinandan
Pal, Samir Kumar
Issue Date: 2009
Publisher: Review of Scientific Instruments
Citation: S. S. Sinha, P. K. Verma, A. Makhal, S. K. Pal, “A Versatile Fiber-optic Coupled System for Sensitive Optical Spectroscopy in Strong Ambient Light”, Review of Scientific Instruments, 80, (2009), 053109
Abstract: In this work we describe design and use of a fiber-optic based optical system for the spectroscopic studies on the samples under the presence of strong ambient light. The system is tested to monitor absorption, emission, and picosecond-resolved fluorescence transients simultaneously with a time interval of 500 ms for several hours on a biologically important sample vitamin B2 under strong UV light. An efficient stray-light rejection ratio of the setup is achieved by the confocal geometry of the excitation and detection channels. It is demonstrated using this setup that even low optical signal from a liquid sample under strong UV-exposure for the picosecond-resolved fluorescence transient measurement can reliably be detected by ultrasensitive microchannel plate photomultiplier tube solid state detector. The kinetics of photodeterioration of vitamin B2 measured using our setup is consistent with that reported in the literature. Our present studies also justify the usage of tungsten light than the fluorescent light for the healthy preservation of food with vitamin B2.
URI: http://hdl.handle.net/123456789/360
Appears in Collections:2009

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